anti rabbit primary  (Bioss)

Journal: Bioactive Materials

Article Title: 3D-MSCs apoptotic bodies-integrated conductive hydrogel mitigates spinal cord injury via immunoregulation and alleviating neuronal pyroptosis

doi: 10.1016/j.bioactmat.2026.01.043

Figure Lengend Snippet: Composite hydrogel promotes the polarization of BV2 cells to M2 types and alleviates PC12 cell pyroptosis in vitro inflammatory environment. (A) Representative western blots showing protein expression of iNOS and Arg-1 in each group, β-actin was utilized as a loading control. (B) Quantitative analysis of relative expression of iNOS and Arg-1. (C) Representative immunofluorescence images of CD68 positive and iNOS positive BV2 cells (scale bar: 20 μm). (D) Representative immunofluorescence images of CD68 positive and Arg-1 positive BV2 cells (scale bar: 20 μm). (E, F) Quantitative analysis of relative fluorescence intensity of iNOS and Arg-1. (G) Representative western blots showing the expression of NLRP3, Caspase-1, IL-1β, ASC, GSDMD-N, and IL-18 protein associated with pyroptosis, β-actin was utilized as a loading control. (H) PI staining of PC12 cells in each group (scale bar, 100 μm). (I) Quantitative analysis of PI staining of PC12 cells (n = 3). (J) Quantitative analysis of relative expression of NLRP3, Caspase-1, IL-1β, ASC, GSDMD-N and IL-18 (n = 3). The data are presented as the means ± SEMs (n = 3); ∗p < 0.05, indicates significant differences; ns, is not significant. Statistical analysis was performed using two-way ANOVA followed by Tukey's multiple comparison test.

Article Snippet: Primary antibodies against NLRP3 (cat. no. 15101) were obtained from Cell Signaling Technology (Beverly, Massachusetts, USA).

Techniques: In Vitro, Western Blot, Expressing, Control, Immunofluorescence, Fluorescence, Staining, Comparison

Journal: Bioactive Materials

Article Title: 3D-MSCs apoptotic bodies-integrated conductive hydrogel mitigates spinal cord injury via immunoregulation and alleviating neuronal pyroptosis

doi: 10.1016/j.bioactmat.2026.01.043

Figure Lengend Snippet: The composite hydrogel inhibits post-SCI pyroptosis in neurons. (A) Immunofluorescence images of residual neurons existing in the anterior horn of the spinal cord (scale bar, 500 μm and 200 μm). (B) Immunofluorescence image of Caspase-1 expression of neurons 3 days after SCI (scale bar, 20 μm). (C) Quantitative analysis of relative fluorescence intensity of Caspase-1 protein expression in neurons within the specified groups (n = 3). (D) Immunofluorescence image of GSDMD-N expression of neurons 3 days after SCI (scale bar, 20 μm). (E) Quantitative analysis of relative fluorescence intensity of GSDMD-N protein expression in neurons within the specified groups (n = 3). (F) Representative western blots showing the expression of NLRP3, Caspase-1, IL-1β, ASC, GSDMD-N, and IL-18 protein 3 days after SCI, GAPDH was utilized as a loading control. (G) Quantitative analysis of relative expression of NLRP3, Caspase-1, IL-1β, ASC, GSDMD-N and IL-18 (n = 3). The data are presented as the means ± SEMs (n = 3); ∗p < 0.05, indicates significant differences; ns, is not significant. Statistical analysis was performed using two-way ANOVA followed by Tukey's multiple comparison test.

Article Snippet: Primary antibodies against NLRP3 (cat. no. 15101) were obtained from Cell Signaling Technology (Beverly, Massachusetts, USA).

Techniques: Immunofluorescence, Expressing, Fluorescence, Western Blot, Control, Comparison

Journal: Clinical and Translational Radiation Oncology

Article Title: MonoHER selectively enhances the radiotherapy response in p53 wild-type breast cancer via stabilization of p53

doi: 10.1016/j.ctro.2026.101147

Figure Lengend Snippet: Effect of monoHER (M) combined with radiation (RT) on γ-H2AX immunofluorescence staining (at 24 h post-irradiation time point) in breast cancer and normal cells. Data are presented as mean ± SEM from three independent experiments (20 cells/experiment). *p < 0.05, **p < 0.01.

Article Snippet: Cells were incubated with a rabbit anti-human γH2AX primary antibody (1:500, Merck, JBW301), followed by detection using goat anti-rabbit Alexa Fluor 488 (1:500, Invitrogen, 11001).

Techniques: Immunofluorescence, Staining, Irradiation

Journal: PLOS One

Article Title: Metformin attenuates TBHP-induced oxidative injury in human lens epithelial cells and is associated with SIRT1/FOXO1-related autophagy

doi: 10.1371/journal.pone.0346822

Figure Lengend Snippet: (a) MDC puncta: TBHP reduces puncta; MET restores/increases puncta (rep. images and quant.; Scale bar, 50 μm). (b-d) IF for Beclin-1, LC3B, p62: TBHP lowers Beclin-1/LC3B and elevates p62; MET reverses these trends; 3-MA attenuates MET-induced changes (rep. images and quant.; Scale bar, 50 μm). (e) WB with densitometry (to GAPDH): TBHP decreases Beclin-1 and LC3B-II/I and increases p62; MET increases Beclin-1 and LC3B-II/I and decreases p62; 3-MA blunts these effects. (f) Flux assay with lysosomal blockade: vs TBHP, MET increases LC3B-II and reduces p62; with CQ, LC3B-II remains high and p62 accumulates; with BafA1, p62 accumulates while LC3B-II shows no further rise under short exposure—consistent with degradation blockade during active autophagosome formation. Data are mean ± SD (n = 3). Stats: one-way ANOVA (Tukey). Exact P values are shown in panels.

Article Snippet: Membranes were blocked with 5% non-fat milk in TBST (Servicebio, China; Cat# G0004) for 1 h at room temperature and incubated overnight at 4 °C with rabbit primary antibodies against Beclin 1 (1:1000), LC3B (1:1000), p62/SQSTM1 (1:10,000), SIRT1 (1:1000), FOXO1 (1:1000), acetyl-FOXO1A (Lys294) (Affinity Biosciences, China; Cat# AF2305; 1:1000), and GAPDH (Affinity Biosciences, China; Cat# T0004).

Techniques: Flux Assay

Journal: PLOS One

Article Title: Metformin attenuates TBHP-induced oxidative injury in human lens epithelial cells and is associated with SIRT1/FOXO1-related autophagy

doi: 10.1371/journal.pone.0346822

Figure Lengend Snippet: (a) ROS: EX-527 partially abrogates the MET-induced reduction (rep. images and quant.; Scale bar, 50 μm). (b) Biochemistry: under TBHP, MET lowers MDA/MPO; EX-527 increases both vs MET (SOD/GSH show no significant difference between MET and MET + EX-527). (c) TUNEL (DAPI): EX-527 diminishes the anti-apoptotic effect of MET (rep. images and quant.; Scale bar, 50 μm). (d-f) IF of Beclin-1, LC3B, p62: the MET-associated pattern (higher Beclin-1 and LC3B, lower p62) is attenuated by EX-527 (Scale bar, 50 μm). (g) WB with densitometry: TBHP decreases Beclin-1 and LC3B-II/I and increases p62; MET reverses these changes; EX-527 blunts the MET effects. Data are mean ± SD (n = 3). Stats: one-way ANOVA (Tukey). Exact P values are shown in panels.

Article Snippet: Membranes were blocked with 5% non-fat milk in TBST (Servicebio, China; Cat# G0004) for 1 h at room temperature and incubated overnight at 4 °C with rabbit primary antibodies against Beclin 1 (1:1000), LC3B (1:1000), p62/SQSTM1 (1:10,000), SIRT1 (1:1000), FOXO1 (1:1000), acetyl-FOXO1A (Lys294) (Affinity Biosciences, China; Cat# AF2305; 1:1000), and GAPDH (Affinity Biosciences, China; Cat# T0004).

Techniques: TUNEL Assay